(1) Closed circular pYe(ARG4)411 DNA was detected in transformant DNA preparations by hybridization to pBR322 DNA and by transformation of E. coli to ampicillin resistance.
(2) The substrates have been used in a spectrophotometric assay of PyEs and in a staining method to locate PyEs on polyacrylamide electrophoresis.
(3) "The bass really vibrates along the floorboards at Pye and one night we were in the middle of a take and it was really moving, going home.
(4) Deputy clerk of the Senate, Richard Pye, has kindly given a translation.
(5) Poets laureate were given sherry until Henry Pye, in 1790, relinquished his butt in exchange for a £27 yearly fee.
(6) Senior G4S personnel, including McCaffrey and centre manager Kevin Pye, who were present on both nights of violence, repeatedly denied that G4S had handed over management of the centre on the evening of 17 February, an allegation reported by ABC TV , based on a guard’s accounts.
(7) A hybrid ColE1 plasmid [pYe(trp5)1], containing a yeast DNA segment that complements auxotrophic point mutations and deletions in the Escherichia coli tryptophan synthetase gene (trpAB), has been isolated.
(8) Saccharomyces cerevisiae, Candida maltosa and Pichia guilliermondii have been transformed by the plasmid pYe(ARG4)411, which contains the S. cerevisiae ARG4 gene inserted into pBR322.
(9) Southern blotting assay of transformant chromosomal DNA indicates that reconstitution of ade2 defective gene is related with its "correction", owing to integration of pYE(ADE2)2 sequence in the vicinity of the mutant locus.
(10) A PYE UNICAM spectrophotometer was used, with KBr pellet technique.
(11) Extracts were evaporated to dryness, whereupon the residues were dissolved in 5 ml of acetone or petroleum ether, and were analysed by gas chromotography (series 104 Pye Unicam gas chromotograph fitted with ECD and TID detectors).
(12) MB • Aberdeen move to within a point of Celtic after comeback at Partick Thistle • Steven Pye: when Aberdeen ruled Scottish football Facebook Twitter Pinterest Mathias Pogba moved from Crawley Town to Partick Thistle last summer but has found goals hard to come by north of the border.
(13) In 1954 he married a teacher, Margaret Pye, from Rhodesia (now Zimbabwe) and moved with her to London.
(14) In particular the experimental work which has been modelled is that of Lanyon, Goodship, Pye and McFie.
(15) The red adenine-dependent mutants ade1 of the yeast Pichia pinus blocked in the VI step of adenine biosynthesis (lack of AIR-carboxylase) and ade2 mutants blocked in the VII step of adenine biosynthesis (lack of SAIKAR-synthase) were transformed with the plasmid pYE(ADE2)2 containing ADE2 gene of Saccharomyces cerevisiae encoding AIR-carboxylase.
(16) Red adenine-dependent mutants of Hansenula polymorpha, Pichia guilliermondii, Williopsis saturnus yeasts have been transformed by the plasmid pYE (ADE2) 2 DNA containing ADE2 gene from Saccharomyces cerevisiae.
(17) But the commission was careful to include a wide range of views, including Pye Jakobsson , who has worked in the Swedish sex industry for several years and has actively campaigned against the criminalisation of punters, and other pro-prostitution activists.
(18) The ionic concentrations were determined using a Pye Unicam SP atomic absorption spectrophotometer.
(19) Since yeast cells transformed by this plasmid poorly secreted RNase into the medium, the plasmid pYE RNAP-Rh was constructed, in which the signal sequence of RNase Rh was replaced by the prepro-sequence of aspartic proteinase-I, one of the extracellular enzymes secreted by R. niveus.
(20) Prepared standards of halothane in oxygen were analysed using a Pye Series 104 gas chromatograph equipped with a heated head flame ionization detector and gas sampling valve.
Pyre
Definition:
(n.) A funeral pile; a combustible heap on which the dead are burned; hence, any pile to be burnt.
Example Sentences:
(1) The nucleotide sequence suggests that pyrE expression is regulated by modulated attenuation, as has been proposed to be the case for both pyrB and pyrE expression in Escherichia coli.
(2) Sequence analysis confirmed this mapping and further showed that fpg is adjacent to rpmBG in the order fpg, rpmGB, pyrE.
(3) The nucleotide sequence of two kilobase pairs (kb) 5' to the orfE-pyrE operon has been determined.
(4) We have used the galK gene, minus its promoter, to quantitate transcription of the orfE--pyrE operon of Escherichia coli in front of and after the intercistronic attenuator.
(5) "If you're in India there'd be a funeral pyre and you get the women throwing themselves on it.
(6) In Australia, the sudden flush of vegetation that followed the loss of large herbivores caused stacks of leaf litter to build up, which became the rainforests' pyre: fires (natural or manmade) soon transformed these lush places into dry forest and scrub .
(7) The incongruously epic promo clip for the cheap-as-chips credit-crunch anthem Dirtee Cash culminates in Dizzee being burnt as a guy on top of a pyre of books (featured texts include TS Eliot's The Wasteland and William Blake's Jerusalem).
(8) Furthermore, the major 3' end of the pyrE mRNA was mapped near a palindromic structure of similarity to the family of repetitive extragenic palindromic sequences, 35 nucleotide residues after stop codon of the pryE gene.
(9) The sequence revealed two open reading frames, orfX and orfY, consisting of 286 and 274 codons, respectively, and having a transcriptional orientation opposite that of the orfE-pyrE operon.
(10) The report by the scientists, released on 11 December, said that the pyre would have required 33 tons of logs, or nearly 1,000 tyres, to reduce 43 bodies to the remains presented as evidence by the attorney general.
(11) The mutation of one mutant of the last class, unlike those of the other nine mutants tested, lay outside the cysE-pyrE segment, in the 90 to 116 min region of the linkage map.
(12) It is like watching a nation busily engaged in heaping up its own funeral pyre …” I don’t think this Ukip poster creators would be insulted by the Enoch Powell comparison Powell foresaw an unchecked inflow of black immigrants creating civil war; this poster tells us absolutely the same thing about the people headed our way, it claims, across borderless Europe.
(13) P1 transduction analyses indicate that the rfa-2 marker is nonallelic with the recently described cysE-pyrE-linked rfaD70 locus.
(14) He said detained members of the gang had led them to the tip where they said they had participated in the massacre and incineration of dozens of young people on a huge funeral pyre that burned for 15 hours on the same night as the students disappeared.
(15) The pyrE gene, encoding the pyrimidine biosynthetic enzyme orotate phosphoribosyltransferase, is the promoter distal gene of the dicistronic orfE-pyrE operon.
(16) The insertion, rec-258, was located between pyrE and dgo at min 82.1 on the current linkage map.
(17) The order of the genes in this region of the E. coli chromosome is: fpg--rpmBG--radC--pyrE.
(18) R-prime plasmids carrying the pyrE-rfa-cysE region of the chromosome of Salmonella typhimurium were isolated by using the vector pULB113 (RP4::mini-Mu).
(19) And there’s a growing natural cremation movement in the UK advocating outdoor funeral pyres.
(20) The results indicate that the NusA protein is required for proper regulation of pyrE gene expression and is involved, together with the NTP pools, in maintaining the coupling between transcription and translation in the pyrE attenuator region by inhibiting RNA chain elongation.