What's the difference between reagent and stain?

Reagent


Definition:

  • (n.) A substance capable of producing with another a reaction, especially when employed to detect the presence of other bodies; a test.

Example Sentences:

  • (1) At 36 h postsurgery, RBCs were examined by 23Na-NMR by using dysprosium tripolyphosphate as a chemical shift reagent.
  • (2) It includes preincubation of diluted plasma with ellagic acid and phospholipids and a starting reagent that contains calcium and a chromogenic peptide substrate for thrombin, Tos-Gly-Pro-Arg-pNA.
  • (3) The assumption was also corroborated using reagents from a family in which DR3 and DQw2 were not found in the usually described linkage.
  • (4) The ADAM derivative of carnitine was separated from decomposition products of the reagent and related compounds such as amino acid derivatives on a silica gel column eluted with methanol-5% aqueous SDS-phosphoric acid (990:10:1).
  • (5) The activity is strongly inhibited by SH-blocking reagents (e.g.
  • (6) Benzyloxycarbonylarginine p-nitrophenyl ester and other activated esters of N-a-sustituted arginine salts may be useful reagents for introduction of trypsin-labile protecting groups into peptide fragments for purpose of polypeptide semi-synthesis.
  • (7) Loss of reduced protein thiols, as measured by binding of the thiol reagent iodoacetic acid to GPD, and loss of GPD enzymatic activity occurred in a dose-dependent manner.
  • (8) Cost-effective immunoassays for the detection of amphetamines, benzodiazepines, and methadone in urine have been developed using Syva EMIT reagents and a Cobas Bio centrifugal analyser.
  • (9) The presence of a previously unreported dipeptide transport mechanism within blood leukocytes and the selective enrichment of the granule enzyme, DPPI, within cytotoxic effector cells of lymphoid or myeloid lineage appear to afford a unique mechanism for the targeting of immunotherapeutic reagents composed of simple dipeptide esters or amides.
  • (10) In the highest concentration tested, two other reagents 2,4-dimethoxybenzylamine and N-acetylimidazole, did not influence the activity of the steroisomers on aorta.
  • (11) On the other hand, the hydrophilic reagents, 1-ethyl-3-[3-(dimethylamino)-propyl]carbodiimide and N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline, did not affect organic cation transport.
  • (12) The histidine-specific reagent diethylpyrocarbonate (DEP) was used to chemically modify TNF.
  • (13) Subgingival plaque was sampled and the presence or absence of the above mentioned bacteria assessed with BANA reagent cards (Perio Scan).
  • (14) Histochemical and immunocytochemical staining of the outgrowths with reagents that depict epithelial, myoepithelial, and lactating alveolar cells (peanut lectin alone, monoclonal and polyclonal antibodies to rat caseins) indicate similar cell compositions and arrangements for all outgrowths irrespective of their source; these are also similar to the mammary glands of the perphenazine-stimulated or lactating hosts.
  • (15) Adhesion and O2- production were also found to be differentially affected by the NADPH oxidase inhibitor diphenylene iodonium, the sulfhydryl reagent N-ethylmaleimide and the A2 agonist adenosine, indicating that these neutrophil responses have various transductional pathways that also depend on the type of stimulus.
  • (16) Either reagent dislocates FAD from the holoenzyme, leaving a characteristic mercaptide derivative of the apoenzyme.
  • (17) Since all five reagents are sensitive to the LA, then the best overall reagent will be the one with the best sensitivity to the most important need for the laboratory (usually heparin monitoring).
  • (18) The enzyme was inhibited by thiol and carbonyl reagents.
  • (19) Radiation inactivation and simple target theory were employed to determine the molecular weight of an insect CNS alpha-bungarotoxin binding component in the presence and absence of a cross-linking reagent, dimethyl suberimate.
  • (20) The probes were biotin treated by chemical modification of the DNA by the peraminating reagent or photochemically.

Stain


Definition:

  • (v. t.) To discolor by the application of foreign matter; to make foul; to spot; as, to stain the hand with dye; armor stained with blood.
  • (v. t.) To color, as wood, glass, paper, cloth, or the like, by processess affecting, chemically or otherwise, the material itself; to tinge with a color or colors combining with, or penetrating, the substance; to dye; as, to stain wood with acids, colored washes, paint rubbed in, etc.; to stain glass.
  • (v. t.) To spot with guilt or infamy; to bring reproach on; to blot; to soil; to tarnish.
  • (v. t.) To cause to seem inferior or soiled by comparison.
  • (v. i.) To give or receive a stain; to grow dim.
  • (n.) A discoloration by foreign matter; a spot; as, a stain on a garment or cloth.
  • (n.) A natural spot of a color different from the gound.
  • (n.) Taint of guilt; tarnish; disgrace; reproach.
  • (n.) Cause of reproach; shame.
  • (n.) A tincture; a tinge.

Example Sentences:

  • (1) The patterns observed were: clusters of granules related to the cell membrane; positive staining localized to portions of the cell membrane, and, less commonly, the whole cell circumference.
  • (2) Within the outflow tract wall, the labelled cells were enmeshed by strands of alcian blue-stained extracellular matrix.
  • (3) The nuclear origin of the Ha antigen was confirmed by the speckled nuclear immunofluorescence staining pattern given by purified antibody to Ha obtained from a specific immune precipitate.
  • (4) The content of the cavities was not stained by any of the immunocytochemical reactions applied.
  • (5) The dependence of fluorescence polarization of stained nerve fibres on the angle between the fibre axis and electrical vector of exciting light (azimuth characteristics) has been considered.
  • (6) Moreover, in DCVC-treated cells the mitochondria could not be stained with rhodamine-123, indicating severe mitochondrial damage and loss of membrane potential.
  • (7) Immunofluorescent staining for HLA-DR showed dermal positivity in 12 of 13 involved- and 9 of 13 uninvolved-skin biopsy specimens from scleroderma patients, compared with only 1 of 10 controls.
  • (8) From these results it was concluded that FITC-Con A staining method applied to smear specimens is more advantageous in the rapidity and the simplicity for tumor cell diagnosis than section specimen method.
  • (9) Further purification of ZAB by filtration through Sephadex G-100 gave a preparation (ZAB2) which contained the common antigen as shown by the cross-reactivity of anti-ZAB2 rat serum with seven stains of N. gonorrhoeae.
  • (10) It has been found that the epidermal staining pattern for ICAM-1 in each of these diseases in distinctive and different in each disease.
  • (11) After either 5 or 10 days of culture with both cytokines, intense immunofluorescent staining for Ia could be identified on the surface of greater than 80-90% of the viable islet cells.
  • (12) In the second comparison, HSV was isolated from 225 of 1,026 (21.9%) specimens and duplicate human foreskin fibroblast cell wells stained at 24 and 72 h were PAP positive in 241 of 1,026 (23.5%).
  • (13) The epithelium of Brunner's gland stained intensely with Ricinus communis agglutinin-I (RCA-I), succinylated-WGA (S-WGA) and wheat-germ agglutinin (WGA), moderately with Bandeirea simplicifolia agglutinin-I (BS-I), Concanavalia ensiformis agglutinin (Con A) peanut agglutinin (PNA) and Ulex europaeus agglutinin-I (UEA-I) and occasionally with Dolichos biflorus agglutinin (DBA), Lens culinaris agglutinin (LCA) and soybean agglutinin (SBA).
  • (14) Using serial section electron microscopic reconstructions as a reference, we have chosen as our standard procedure a method that maximizes both the preservation of the cytoskeleton and the proportion of cells staining, while minimizing the degree of nonspecific staining.
  • (15) One major band with a molecular weight of 12,000 was detected by autofluorography and coincided with the Coomassie staining band of apocytochrome c from S. cerevisiae.
  • (16) Pitlike surface structures seen in negatively stained whole cells and thin sections were correlated with periodically spaced perforations of the rigid sacculus.
  • (17) In the present study, 125 oesophageal biopsies obtained under direct vision at endoscopy from 22 patients with Barrett's oesophagus were systematically studied using fluorescence and peroxidase antiperoxidase single and double-staining immunocytochemical methods employing highly specific antibodies to localize the following peptide-containing cell types in Barrett's mucosa: gastrin, somatostatin, gastric inhibitory polypeptide, motilin, neurotensin and pancreatic glucagon.
  • (18) The rate of nuclei stained by Pr-122 is different from that of Pr-192 in both growing and quiescent cultures.
  • (19) This light microscopic comparison of viable FDA- and nonviable PI-stained cysts of G. muris demonstrates that 2 types of cysts can be distinguished and implies that structural differences can be used to identify these subpopulations of cysts.
  • (20) Benign and malignant epithelial and soft tissue tumors of the skin were usually negatively stained with MoAb HMSA-2.