(1) Twelve solutions of 0.1 M boric acid and varying glycerol concentration were used to vary pH in a twelve-compartment commercial recycling isoelectric focusing (RIEF) system.
(2) Continuous flow zone electrophoresis (CFE) and recycling isoelectric focusing (RIEF) are two of the alternative formats for fluid phase preparative isolation of biological products in liquid separation media.
(3) RIEF clearly separated each protein with no detectable contamination.
(4) These data demonstrate the superiority of RIEF over CFE for resolution of protein mixtures having only minor charge differences.
(5) Gel filtration chromatography removed most of the remaining, higher molecular weight contaminants of the RIEF-purified enzyme.
(6) Mixtures of 1) cytochrome c, myoglobin, and ovalbumin or 2) beta-lactoglobulin and ovalbumin were used to evaluate the resolving capabilities of CFE and RIEF.
(7) The ground-based McDonnell Douglas CFE and RIEF were compared for the ability to resolve mixtures of proteins with known charge differences.
(8) RIEF may be more efficient due to the documented electrodissociation of noncovalent protein:protein complexes which occurs during RIEF separations.
(9) It is concluded that the borate-glycerol system can be adequately stabilized in RIEF for isoelectric purification of certain proteins.
(10) Fibrolase, a blood clot-lysing enzyme, was isolated from the venom of the snake Agkistrodon contortrix contortrix using preparative scale isoelectric focusing in the recycling isoelectric focusing (RIEF) apparatus.
(11) Both CFE and RIEF apparently separated the components of both mixtures into individual peaks, separated by fractions which contained little or no detectable protein.
Rife
Definition:
(a.) Prevailing; prevalent; abounding.
(a.) Having power; active; nimble.
Example Sentences:
(1) As an organisation rife with white privilege, Peta has the luxury of not having to consider the horror that such imagery would evoke.
(2) Independent experts warn that rumours and deliberate misinformation about the regime are rife, partly because it is impossible to verify or disprove most stories about the tightly controlled country's elite.
(3) Crosta said the lack of internet access in many parts of the world where wildlife crime is rife was not a major barrier to success, because WildLeaks is aimed at exposing the key players in the international crime networks, not the low-level operatives on the ground.
(4) The City is rife with gambling addicts whose habits contribute to a risk-prone culture of the sort which helped Kweku Adoboli lose UBS £1.5bn, according to one London trader.
(5) Perhaps monstering earns underdog sympathy, with contempt for the press as rife as contempt for conventional politics.
(6) She wants it to be a smooth, constructive, orderly process.” With speculation rife about how Britain plans to conduct the negotiations, Tusk wants to avoid a discussion and will not invite other EU leaders to respond.
(7) Concern – which was already rife among Britons living and working in EU countries about the effect on their lives if Britain were to leave the single market and reject freedom of movement – has been compounded by last week’s UK general election result.
(8) James Murdoch, the New York Times and Sienna Miller The New York Times published an report in September 2010 claiming "a dozen" reporters had said hacking was rife at the News of the World.
(9) On Sunday, Jones tweeted about "heavy shelling and other exchanges" of fire in the vicinity of the embassy and speculation about the potential evacuation had been rife at the State Department for more than a week.
(10) It is all too easy to show that RT’s coverage is rife with conspiracy theories and risible fabrications: one programme showed fake documents intended to prove that the US was guiding the Ukrainian government to ethnically cleanse Russian speakers from western Ukraine.
(11) Speculation is rife that international aid was dependent on Greece following through on agreements to buy military hardware from Germany and France.
(12) Competition among software providers is rife, and it can be difficult to determine which option is best suited for particular organisational and contextual needs.
(13) Critics have long charged that Alibaba’s Taobao online marketplace, one of the world’s largest shopping sites with 7 million sellers offering 800 million items, is rife with fake goods.
(14) The bill was rife with the sentiment that Snap recipients are largely lazy, unemployed, or underemployed, and that the solution to Snap's expansion was to force its recipients to take jobs.
(15) Even so, Steve Gibson, the club’s owner apparently came very close to sacking the Spaniard and rumours are rife that Karanka might not be around next season with some believing Nigel Pearson could step in.
(16) Malaria is rife: children under seven and pregnant women cannot be sent there because of malarial issues.
(17) The clashes occurred close to the village of Elbeyli, a small border town rife with smugglers to which the Turkish military has sent reinforcements in recent weeks.
(18) In the London agencies where she worked in the 80s, overt sexism was rife, but Gallop says she didn’t notice “because that was the way things were.
(19) Use of these new damaging and powerful forms of synthetic cannabinoids is rife in our prisons and by homeless people, with estimates of up to 50 deaths last year .
(20) But gagging clauses, self-censorship and dread of speaking out is rife.