What's the difference between safranin and stain?

Safranin


Definition:

  • (n.) An orange-red dyestuff extracted from the saffron.
  • (n.) A red dyestuff extracted from the safflower, and formerly used in dyeing wool, silk, and cotton pink and scarlet; -- called also Spanish red, China lake, and carthamin.
  • (n.) An orange-red dyestuff prepared from certain nitro compounds of creosol, and used as a substitute for the safflower dye.

Example Sentences:

  • (1) Stage IV cells contain only safranin-positive granules.
  • (2) In 13- and 15-day embryos, mast cells showed a pale metachromasia with toluidine blue, and stained blue with Alcian Blue-Safranin O (AB-S).
  • (3) At 30 and 60 days, an S-100 positive band of cells separated a deep safranin-O positive hypertrophic layer from a fibrocellular surface layer.
  • (4) The increase of cAMP level also affected the maturation of mast cells, as the ratio of cells of mixed granulation increased, compared to the alcian blue- and safranin-present model inhibited degranulation.
  • (5) Astra blue (AB) safranine stained highly significantly more mast cells than did toluidine blue.
  • (6) There was a profound loss of safranin-O stainability in the nucleus pulposus, annulus fibrosus and cartilagenous endplate.
  • (7) Other staining techniques assessed were the modified Ziehl-Neelsen, safranin-methylene blue and auramine-phenol fluorescence.
  • (8) A zone of safranin O depletion was present in the ventral anulus fibrosus adjacent to the nucleus pulposus in all treated discs, indicating proteoglycan loss.
  • (9) The distribution and localization of S-100 protein was compared with safranin-O staining and H and E morphology in relatively unaffected, degenerative, and osteophytic regions of human articular cartilage from 26 joints obtained at the time of total joint replacement for osteoarthritis.
  • (10) Both safranin O and Giemsa were suitable nonfluorescent staining techniques; lomofungin was not.
  • (11) After 8-12 weeks, however, many subserosal mast cells became positive for berberine sulfate and safranin.
  • (12) Defects in treated joints contained Safranin O staining material that was histologically similar to a disorganized hyaline cartilage.
  • (13) Intestinal MC stained with the same dyes as oral MC except for 0.005% toluidine blue, berberine sulfate, and safranin.
  • (14) A new microspectrophotometric method was developed for quantitation of glycosaminoglycans with Safranin O dye in articular cartilage matrix.
  • (15) It is concluded that the use of safranine to monitor the changes in membrane potential during Ca2+ transport by mitochondria should be avoided or special care be taken.
  • (16) The selectivity of myoepithelial cell staining is enhanced by oxidation of sections, nuclear staining by Safranin-O, and differentiation with Tartrazine.
  • (17) Ferric ion-ferrocyanide staining and safranin-0-counterstaining of neocortical tissue from cats with GM1 gangliosidosis have established that pyramidal neuron meganeurites occur proximal to axonal initial segments and that they are distinct from axonal spheroids.
  • (18) Cytochrome oxidase reconstituted vesicles, supplemented with ascorbate and cytochrome c. induce large spectral changes of the positive dye safranine, reversed by uncouplers and inhibitors of respiration.
  • (19) Histologic specimens of the articular cartilage were stained with Safranin-O to assess proteoglycans-enhanced chrondrocyte function, and the synovium was stained with pentachrome.
  • (20) Synaptosomes were severely energy-deprived, because anaerobic glycolysis was inhibited by 90% from the aerobic level and mitochondrial membrane potential dropped below the limit that could be detected by the safranine method.

Stain


Definition:

  • (v. t.) To discolor by the application of foreign matter; to make foul; to spot; as, to stain the hand with dye; armor stained with blood.
  • (v. t.) To color, as wood, glass, paper, cloth, or the like, by processess affecting, chemically or otherwise, the material itself; to tinge with a color or colors combining with, or penetrating, the substance; to dye; as, to stain wood with acids, colored washes, paint rubbed in, etc.; to stain glass.
  • (v. t.) To spot with guilt or infamy; to bring reproach on; to blot; to soil; to tarnish.
  • (v. t.) To cause to seem inferior or soiled by comparison.
  • (v. i.) To give or receive a stain; to grow dim.
  • (n.) A discoloration by foreign matter; a spot; as, a stain on a garment or cloth.
  • (n.) A natural spot of a color different from the gound.
  • (n.) Taint of guilt; tarnish; disgrace; reproach.
  • (n.) Cause of reproach; shame.
  • (n.) A tincture; a tinge.

Example Sentences:

  • (1) The patterns observed were: clusters of granules related to the cell membrane; positive staining localized to portions of the cell membrane, and, less commonly, the whole cell circumference.
  • (2) Within the outflow tract wall, the labelled cells were enmeshed by strands of alcian blue-stained extracellular matrix.
  • (3) The nuclear origin of the Ha antigen was confirmed by the speckled nuclear immunofluorescence staining pattern given by purified antibody to Ha obtained from a specific immune precipitate.
  • (4) The content of the cavities was not stained by any of the immunocytochemical reactions applied.
  • (5) The dependence of fluorescence polarization of stained nerve fibres on the angle between the fibre axis and electrical vector of exciting light (azimuth characteristics) has been considered.
  • (6) Moreover, in DCVC-treated cells the mitochondria could not be stained with rhodamine-123, indicating severe mitochondrial damage and loss of membrane potential.
  • (7) Immunofluorescent staining for HLA-DR showed dermal positivity in 12 of 13 involved- and 9 of 13 uninvolved-skin biopsy specimens from scleroderma patients, compared with only 1 of 10 controls.
  • (8) From these results it was concluded that FITC-Con A staining method applied to smear specimens is more advantageous in the rapidity and the simplicity for tumor cell diagnosis than section specimen method.
  • (9) Further purification of ZAB by filtration through Sephadex G-100 gave a preparation (ZAB2) which contained the common antigen as shown by the cross-reactivity of anti-ZAB2 rat serum with seven stains of N. gonorrhoeae.
  • (10) It has been found that the epidermal staining pattern for ICAM-1 in each of these diseases in distinctive and different in each disease.
  • (11) After either 5 or 10 days of culture with both cytokines, intense immunofluorescent staining for Ia could be identified on the surface of greater than 80-90% of the viable islet cells.
  • (12) In the second comparison, HSV was isolated from 225 of 1,026 (21.9%) specimens and duplicate human foreskin fibroblast cell wells stained at 24 and 72 h were PAP positive in 241 of 1,026 (23.5%).
  • (13) The epithelium of Brunner's gland stained intensely with Ricinus communis agglutinin-I (RCA-I), succinylated-WGA (S-WGA) and wheat-germ agglutinin (WGA), moderately with Bandeirea simplicifolia agglutinin-I (BS-I), Concanavalia ensiformis agglutinin (Con A) peanut agglutinin (PNA) and Ulex europaeus agglutinin-I (UEA-I) and occasionally with Dolichos biflorus agglutinin (DBA), Lens culinaris agglutinin (LCA) and soybean agglutinin (SBA).
  • (14) Using serial section electron microscopic reconstructions as a reference, we have chosen as our standard procedure a method that maximizes both the preservation of the cytoskeleton and the proportion of cells staining, while minimizing the degree of nonspecific staining.
  • (15) One major band with a molecular weight of 12,000 was detected by autofluorography and coincided with the Coomassie staining band of apocytochrome c from S. cerevisiae.
  • (16) Pitlike surface structures seen in negatively stained whole cells and thin sections were correlated with periodically spaced perforations of the rigid sacculus.
  • (17) In the present study, 125 oesophageal biopsies obtained under direct vision at endoscopy from 22 patients with Barrett's oesophagus were systematically studied using fluorescence and peroxidase antiperoxidase single and double-staining immunocytochemical methods employing highly specific antibodies to localize the following peptide-containing cell types in Barrett's mucosa: gastrin, somatostatin, gastric inhibitory polypeptide, motilin, neurotensin and pancreatic glucagon.
  • (18) The rate of nuclei stained by Pr-122 is different from that of Pr-192 in both growing and quiescent cultures.
  • (19) This light microscopic comparison of viable FDA- and nonviable PI-stained cysts of G. muris demonstrates that 2 types of cysts can be distinguished and implies that structural differences can be used to identify these subpopulations of cysts.
  • (20) Benign and malignant epithelial and soft tissue tumors of the skin were usually negatively stained with MoAb HMSA-2.

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