(1) The amino acid sequence deduced from the DNA sequence was determined for the plasmid-coded and the ScrY porin coded in the chromosome of Klebsiella pneumoniae.
(2) Primer extension analysis and site-directed mutagenesis were used to identify the precise location of the promoter of scrY, scrA, and scrB.
(3) This increase in sucrose permeability provided strong evidence that the ScrY protein functions as a sucrose porin.
(4) A putative cyclic AMP receptor protein binding site centered 72.5 bp upstream of the start point of transcription of scrY appeared to be essential for full activity of the scrY promoter.
(5) Furthermore, the presence of ScrY restored growth on maltodextrins in cells devoid of LamB, thus complementing the lack of this maltoporin.
(6) Reconstitution experiments with lipid bilayer membrane demonstrated that ScrY formed ion-permeable channels with properties very similar to those of general diffusion pores of enteric bacteria.
(7) This sounds shocking, but dig a little deeper and some of this soul-scrying voodoo becomes slightly less terrifying.
(8) The binding of different sugars to ScrY and LamB of E. coli is discussed with respect to the kinetics of sugar movement through the channel.
(9) A frameshift mutation in the scrY gene resulted in a dramatic decrease in sucrose transport with no effect on in vitro phosphorylation activity associated with enzyme IISer.
(10) There was 23% amino acid sequence identity between the ScrY protein and LamB, a maltose porin from Escherichia coli.
(11) One of the different gene products of the plasmid is the outer membrane protein, ScrY.
(12) During the molecular analysis of a plasmid-coded sucrose metabolic pathway of enteric bacteria, a gene, scrY, was found whose product, ScrY, had all the properties of a bacterial porin (Schmid et al., 1988).
(13) In ScrR+ cells, readthrough transcription from the putative scrK promoter into scrY accounted for less than 10% of scrY expression.
(14) The scrY gene, part of the pUR400-borne sucrose regulon, appeared to be transcribed from its own promoter, with the transcriptional start site located 58 bp upstream from the initiation codon.
(15) The four genes form an scr operon (gene order, scrK scrY scrA scrB, transcription from K to B), regulated by a repressor (gene scrR, 37 kD) and inducible by sucrose, fructose and fructose-containing oligosaccharides.
(16) Gene scrK apparently codes for an intracellular and ATP-dependent fructokinase (39 kD), while scrY seems to code for a sucrose porin (58 kD) in the outer cell membrane.
(17) The rate of diffusion of sucrose was 96 times greater than the rate of diffusion of lactose or maltose in liposomes containing the ScrY protein.
Witch
Definition:
(n.) A cone of paper which is placed in a vessel of lard or other fat, and used as a taper.
(n.) One who practices the black art, or magic; one regarded as possessing supernatural or magical power by compact with an evil spirit, esp. with the Devil; a sorcerer or sorceress; -- now applied chiefly or only to women, but formerly used of men as well.
(n.) An ugly old woman; a hag.
(n.) One who exercises more than common power of attraction; a charming or bewitching person; also, one given to mischief; -- said especially of a woman or child.
(n.) A certain curve of the third order, described by Maria Agnesi under the name versiera.
(n.) The stormy petrel.
(v. t.) To bewitch; to fascinate; to enchant.
Example Sentences:
(1) I fear that I will have to go through another witch-hunt in order to apply for this benefit."
(2) "I have been an evil witch, but now I can set light to the house and die happy."
(3) The experience of having had intercourse with the devil has in the past been regarded as evidence that the individual is a witch.
(4) Smith, a climate change sceptic who has also subpoenaed government scientists’ communications, has accused the attorney generals of a political witch-hunt and for causing a “chilling impact on scientific research and development”.
(5) In 2005, four years after Adam's body was found, two women and a man were convicted of child cruelty for torturing and threatening to kill an orphaned refugee who they claimed was a witch.
(6) The Witch Is Dead, the Wizard of Oz song which became the focus of an anti-Thatcher campaign on Facebook, was not just about where it would chart – but how much of it the BBC would play.
(7) A couple have been jailed for life for torturing and drowning a teenage boy they accused of being a witch.
(8) Leave voters, including a soldier, a mother expecting a “Brexit baby” due nine months after the vote, a rare chicken breeder, a witch, and a hammer-wielding Nigel Farage fan, have all been chosen to represent the various faces of Brexit on a new vase by the artist Grayson Perry .
(9) On Christmas Day 2010, Kristy's killer spoke to the boy's father, Pierre, accusing the 15-year-old of being a witch and threatening to kill him.
(10) Social unrest has become more and more likely, leading to an increasingly bold witch-hunt by the government against opposition voices .
(11) Lee denied the charges, saying he had never heard of the Revolutionary Organisation and denouncing the trial as a politically motivated witch-hunt by intelligence officials.
(12) The government has launched a separate royal commission into alleged union corruption, which unions have argued is a politically motivated “witch hunt”.
(13) Sure, the season’s story, which focuses on Vanessa Ives’s struggle to decode the “memoirs of the devil” and fight a hissing viper pit of Lucifer’s witches, may be pure pulp burlesque, but that’s just the first layer of Penny Dreadful’s charm.
(14) I could be the most beautiful drag queen in the world and the most evil witch of a person.
(15) Human rights campaigners have called on South Korea’s military to end its “witch-hunt” against gay servicemen, after an investigation into dozens of men prompted debate among presidential candidates over the country’s poor record on LGBT rights.
(16) "If we don't push home the idea that calling a child a witch will have grave consequences, then we will continue to have these kind of cases," said Ariyo.
(17) At one point, Evans was accused of bullying staff 20 years ago – a claim he said was ridiculous and the result of a witch-hunt.
(18) Season two crafted complex characters racked with existential ambivalence – heroines marked for the abyss, fragile, flammable outcasts and desolate prodigies, all of whose private pain was as palpable as the crimson bloodbath head witch Evelyn Poole soaks in.
(19) After working in a second-rate singing act with her older sisters and changing her name from Frances Gumm to Judy Garland, she was taken to Hollywood at the age of 13 by her fiercely ambitious mother (whom she later called "the real Wicked Witch of the West").
(20) He tried to capture its character – which he described as a “diabolical contraption, a dusty hunk of electric and mechanical hardware that reminded me of the disturbing 1950’s Quatermass science fiction television series” – in a near-lifesize two metre by three metre Portrait of a Dead Witch, which he also intended as a joke about the contemporary craze for computer-generated art.