(1) The resulting vector, pHSG683, confers chloramphenicol resistance, enforces SmS on strAR and supE- host bacteria, and has multiple cloning sites within the coding region of synthetic rpsL gene.
(2) When pHSG683 DNA was prepared from strAR and sup+ cells grown in tryptophan-rich medium with Cm and Sm, less than 10(-6) plasmids failed to enforce SmS on strAR and supE- cells in tryptophan-less medium with Cm.
(3) The lung volume at which airway closure occurred in pregnancy and the post-partum period did not change in relation to the vital capacity whereas the expiratory reserve volume did, so that airway closure became imminent in the pregnant women during tidal ventilation, especially whilst in the suping position.
(4) We show here that the mutation in rodA is a single-base change from a glutamine to a chain termination (amber) codon, and that an amber suppressor (supE) present in the strain restores the ability to produce a reduced level of normal RodA protein.
(5) The lethal phenotype was expressed specifically in supE (glutamine-inserting) host strains and not in Sup+ strains, because the mutant genes, by design, also possessed an amber mutation derived from a glutamine codon.
(6) About 90% of the Trp+ revertants induced by methylnitrosocyanamide were due to mutations in suppressor genes, and 85% of the suppressor mutations occurred in gene supE.
(7) This can be eliminated by using a supE-strain as MG1655 and by site-directed mutagenesis of the cDNA to create an in frame UAA stop codon.
(8) All three mischarging mutant enzymes still retain a certain degree of tRNA specificity; in vivo they acylate supE glutaminyl tRNA (tRNA(Gln] and supF tRNA(Tyr) but not a number of other suppressor tRNA's.
(9) The asuA1 and asuA2 mutations are linked to trp, reduce suppression by supE approximately twofold, and affect a variety of suppressors.
(10) When amber suppressor mutations at the supD, supE, or supF loci were introduced into such sup0 osmodefective strains, they became osmotolerant and gained the ability to accumulate trehalose in response to elevated medium osmolarity.
(11) The two independently isolated suppressors behaved as supD and supE mutants, respectively, of E. coli.
(12) High levels of expression of I-FABP mRNA in supE+ strains of E. coli, such as JM101, is associated with suppression of termination at its UGA stop codon.
(13) Our results indicate that the supE glutamine tRNA context can tolerate multiple mutations (including mutation of the discriminator and first base-pair) and still remain predominantly glutamine-accepting.
(14) Of the 15 mutants generated by this method, none are suppressible by supD, supE, or supF.
(15) The effects of streptomycin and neomycin on the phenotypic suppression of frameshift mutations in the lacZ gene of Escherichia coli and on the efficiency of suppression of amber mutations in T4 phage by the informational supE tRNA nonsense suppressor were compared.
(16) Suppressor analysis by using BE1047 (trpambphooch) revealed that the most frequently occurring reversion was due to a mutation in suppressor gene, supE.
(17) Plaque-forming phages carrying the E. coli trp, his, cysB, thyA, supD, supE, sup F, hsd, tna and lig genes have been isolated.
(18) Or perhaps Aquaman could pop his head unexpectedly above the waterline and reveal the existence of a previously unimagined submerged society of mer-men while Supes is having a quiet day off fishing.
(19) Phage of the insertion mutant, M13mp19-NheI, produced light blue plaques on SupE strains because of the introduced amber codon.
(20) In all three, the relaxed phenotype was suppressed by supD, supE, supF or sup6.
(superl.) Certain to find or retain; as, to be sure of game; to be sure of success; to be sure of life or health.
(superl.) Fit or worthy to be depended on; certain not to fail or disappoint expectation; unfailing; strong; permanent; enduring.
(superl.) Betrothed; engaged to marry.
(superl.) Free from danger; safe; secure.
(adv.) In a sure manner; safely; certainly.
Example Sentences:
(1) I'm not sure Tolstoy ever worked out how he actually felt about love and desire, or how he should feel about it.
(2) We want to be sure that the country that’s providing all the infrastructure and support to the business is the one that reaps the reward by being able to collect the tax,” he said.
(3) To be sure, the demonstration of pulmonary aspiration with GRS had little influence on patient selection and response to therapy.
(4) If you want to become a summit celebrity be sure to strike a pose whenever you see the ENB photographer approaching.
(5) Surely Michael wasn't saying he agreed with what Blair is doing?
(6) To be sure, when Russia withdrew Cuba's only deterrent against ongoing US attack with a severe threat to proceed to direct invasion and quietly departed from the scene, the Cubans would be infuriated – as they were, understandably.
(7) What happened in the past was that if smugglers are sure that European boats are patrolling very close to the Libyan coast, then traffickers use this opportunity to advertise, and say to potential irregular migrants: ‘You will be sure to reach the European coast.
(8) But no one was sure, and in this information vacuum the virus reached nearby towns and crossed borders.
(9) If this is the only issue, flight would be fine, but need to make sure that it isn’t symptomatic of a more significant upstream root cause.” Elon Musk (@elonmusk) Btw, 99% likely to be fine (closed loop TVC wd overcome error), but that 1% chance isn't worth rolling the dice.
(10) While visitors amble freely around the newly refurbished inside – the Pierhead is sure and steadfast in its role outside as the drastic red building, emblazoning the landscape of Cardiff Bay in all its regal beauty.
(11) The letters, seen by Guardian Money, state that the French-owned company is conducting a review of customer records to make sure all its information is up to date.
(12) "If I hadn't scored that goal, I might still have ended up playing in Italy [Platt went on to play for Bari, Juventus and Sampdoria] but, realistically, I'm sure it was the catalyst.
(13) Although it never really has a sense of fun and burns with ill-focused anger, The Paperboy represents a kind of triumph, surely, even if it's just in getting such high-profile actors to do such low-down deeds.
(14) Their brutality seems to have been fairly even-handed, or if it wasn't, the men surely suffered enough not to be presented as the winners of the atrocity.
(15) If figurative language is defined as involving intentional violation of conceptual boundaries in order to highlight some correspondence, one must be sure that children credited with that competence have (1) the metacognitive and metalinguistic abilities to understand at least some of the implications of such language (Lakoff & Johnson, 1980; Nelson, 1974; Nelson & Nelson, 1978), (2) a conceptual organization that entails the purportedly violated conceptual boundaries (Lange, 1978), and (3) some notion of metaphoric tension as well as ground.
(16) Doreen Lawrence to speak at conference on police spying, corruption and racism Read more Mick Creedon, the Derbyshire Chief Constable who is leading the police’s internal investigation into the SDS, said the public inquiry “will help us with the work that is already underway to make sure that the unacceptable behaviour of some officers in the past never happens again”.
(17) According to his blog, he's been acting on the advice of a friend and pursuing a course of "silence, exile and cunning", but I'm not sure a couple of years of not giving interviews to Heat qualifies.
(18) Asked by Marr if he knew if Ashcroft paid tax in this country, Hague said:" I'm sure he fulfils the obligations that were imposed on him at the time he became …" Marr: "Have you asked him?"
(19) Financial experts aren't immediately sure what to make of the report, but one theory is that the figure includes the 'profits' the European Central Bank has made by buying Greek debt at distressed levels since the crisis began: econhedge (@econhedge) suggestion that this is planned EUR31.5b+ECB profits.
(20) This is a very nice drug and I’m sure Merck are feeling very pleased with themselves.” Matt Kennedy, who led the trial at Merck, said: “Today there are very limited therapeutic options available for people with Alzheimer’s disease, and those that exist provide only short-term improvement to the cognitive and functional symptoms.